Scientists worldwide use the miRNA overexpression method in their experiments. Typically, the accuracy of the results is verified using polymerase chain reaction (PCR). However, this method was not ...
Diana Maltseva, at el. Incautious design of shRNAs for stable overexpression of miRNAs could result in generation of undesired isomiRs, Biochimica et Biophysica Acta (BBA) — Gene Regulatory Mechanisms ...