Many factors influence assay design, such as amplicon length, GC content, sequence specificity, and melting temperature, all of which impact the final target sequence, primers, and probes. Flawed ...

Applied Biosystems TaqPath assays are the first choice for probe-based qPCR assays that have been specifically designed for late-stage biotechnology and pharmaceutical applications.

Further, they aligned primer and probe sequences and their reverse complements in the seven real-time PCR assays against 1,779 MPXV genomes and computed the percentage of genomes with a 100% match.

Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and ...

Exiqon is now able to address this need by offering custom assays based on LNA (Locked Nucleic Acid) enabled real-time PCR.

Objectives: Detection of Neisseria gonorrhoeae by commercial and in-house-based assays has been hampered by false-positive and false-negative results. The current study describes a sensitive and ...